Dr. Robert Winkler

Position:  Investigador Cinvestav 3C, SNI 3

Telephone: +52 (462) 623 9635


Laboratory of biochemical and instrumental analysis




Research » Biotechnology and Biochemistry » Professors/Researchers » Dr. Robert Winkler » Publications

Author Profiles

Google ScholarORCID, puplons, ResearcherIDResearchGate, Scopus


1. Atriztán-Hernández, K., Moreno-Pedraza, A., Winkler, R., Markow, T. & Herrera-Estrella, A. Trichoderma atroviride from predator to prey: Role of the MAPK Tmk3 in fungal chemical defense against fungivory by Drosophila melanogaster larvae. Appl. Environ. Microbiol. AEM.01825–18 (2018). doi:10.1128/AEM.01825-18

2. Montero-Vargas, J. M., Casarrubias-Castillo, K., Martínez-Gallardo, N., Ordaz-Ortiz, J. J., Délano-Frier, J. P. & Winkler, R. Modulation of steroidal glycoalkaloid biosynthesis in tomato (Solanum lycopersicum) by jasmonic acid. Plant Science 277, 155–165 (2018).

3. Puertas-Mejía, M. A., Gutierrez-Villegas, M. I., Mejía-Giraldo, J. C., Winkler, R., Rojano, B., Puertas-Mejía, M. A., Gutierrez-Villegas, M. I., Mejía-Giraldo, J. C., Winkler, R. & Rojano, B. In vitro UV absorption properties and radical scavenging capacity of Morella parvifolia (Benth.) Parra-Os. Extracts. Brazilian Journal of Pharmaceutical Sciences 54, (2018).

4. Barraza, A., Coss-Navarrete, E. L., Vizuet-de-Rueda, J. C., Martínez-Aguilar, K., Hernández-Chávez, J. L., Ordaz-Ortiz, J. J., Winkler, R., Tiessen, A. & Alvarez-Venegas, R. Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.). Plant Science 274, 45–58 (2018).

5. López-Castillo, L. M., Silva-Fernández, S. E., Winkler, R., Bergvinson, D. J., Arnason, J. T. & García-Lara, S. Postharvest insect resistance in maize. Journal of Stored Products Research 77, 66–76 (2018).

6. Martínez-Jarquín, S., Herrera-Ubaldo, H., Folter, S. de & Winkler, R. In vivo monitoring of nicotine biosynthesis in tobacco leaves by low-temperature plasma mass spectrometry. Talanta 185, 324–327 (2018).

7. López-Castillo, L. M., Díaz Flores-Rivera, M. F., Winkler, R. & García-Lara, S. Increase of peroxidase activity in tropical maize after recurrent selection to storage pest resistance. Journal of Stored Products Research 75, 47–55 (2018).

8. Vergara, F., Itouga, M., Becerra, R. G., Hirai, M., Ordaz-Ortiz, J. J. & Winkler, R. Funaria hygrometrica Hedw. elevated tolerance to D2O: its use for the production of highly deuterated metabolites. Planta 247, 405–412 (2018).

9. Gamboa-Becerra, R., Montero-Vargas, J. M., Martínez-Jarquín, S., Gálvez-Ponce, E., Moreno-Pedraza, A. & Winkler, R. Rapid Classification of Coffee Products by Data Mining Models from Direct Electrospray and Plasma-Based Mass Spectrometry Analyses. Food Analytical Methods 10, 1359–1368 (2017).

10. Martínez-Jarquín, S. & Winkler, R. Low-temperature plasma (LTP) jets for mass spectrometry (MS): Ion processes, instrumental set-ups, and application examples. TrAC Trends in Analytical Chemistry 89, 133–145 (2017).

11. Tiessen, A., Cubedo-Ruiz, E. A. & Winkler, R. Improved Representation of Biological Information by Using Correlation as Distance Function for Heatmap Cluster Analysis. American Journal of Plant Sciences 8, 502–516 (2017).

12. Martínez-Jarquín, S., Moreno-Pedraza, A., Cázarez-García, D. & Winkler, R. Automated chemical fingerprinting of Mexican spirits derived from Agave (tequila and mezcal) using direct-injection electrospray ionisation (DIESI) and low-temperature plasma (LTP) mass spectrometry. Analytical Methods 9, 5023–5028 (2017).

13. Krewinkel, A. & Winkler, R. Formatting Open Science: agilely creating multiple document formats for academic manuscripts with Pandoc Scholar. PeerJ Computer Science 3, 1–23 (2017).

14. Sánchez-Roque, Y., Ayora-Talavera, G., Rincón-Rosales, R., Gutiérrez-Miceli, F. A., Meza-Gordillo, R., Winkler, R., Gamboa-Becerra, R., Rosario Ayora-Talavera, T. del & Ruiz-Valdiviezo, V. M. The Flavonoid Fraction from Rhoeo discolor Leaves Acting as Antiviral Against Influenza A Virus. Records of Natural Products 11, 532–546 (2017).

15. Solorio-Rodríguez, A., Escamilla-Rivera, V., Uribe-Ramírez, M., Chagolla, A., Winkler, R., Garcia-Cuellar, C. & De Vizcaya-Ruiz, A. A comparison of human and mouse protein corona profiles of functionalized SiO2 nanocarriers. Nanoscale 9, 13651–13660 (2017).

16. Cázarez-García, D., Loustalot-Laclette, M. R., Markow, T. A. & Winkler, R. Lipidomic profiles of Drosophila melanogaster and cactophilic fly species: models of human metabolic diseases. Integrative Biology 9, 885–891 (2017).

17. Rendón-Anaya, M., Montero-Vargas, J. M., Saburido-Álvarez, S., Vlasova, A., Capella-Gutierrez, S., Ordaz-Ortiz, J. J., Aguilar, O. M., Vianello-Brondani, R. P., Santalla, M., Delaye, L., Gabaldón, T., Gepts, P., Winkler, R., Guigó, R., Delgado-Salinas, A. & Herrera-Estrella, A. Genomic history of the origin and domestication of common bean unveils its closest sister species. Genome Biology 18, 60 (2017).

18. Winkler, R. Popper and the Omics. Frontiers in Plant Science 7, 1–3 (2016).

19. Martínez-Jarquín, S., Moreno-Pedraza, A., Guillén-Alonso, H. & Winkler, R. Template for 3D printing a low-temperature plasma probe. Analytical Chemistry 88, 6976–6980 (2016).

20. Mejía‐Giraldo, J. C., Winkler, R., Gallardo, C., Sánchez‐Zapata, A. M. & Puertas‐Mejía, M. A. Photoprotective Potential of Baccharis antioquensis (Asteraceae) as Natural Sunscreen. Photochemistry and Photobiology 92, 742–752 (2016).

21. López-Castillo, L. M., Jiménez-Sandoval, P., Baruch-Torres, N., Trasviña-Arenas, C. H., Díaz-Quezada, C., Lara-González, S., Winkler, R. & Brieba, L. G. Structural Basis for Redox Regulation of Cytoplasmic and Chloroplastic Triosephosphate Isomerases from Arabidopsis thaliana. Frontiers in Plant Science 7, 1–17 (2016).

22. García-Flores, M., Juárez-Colunga, S., García-Casarrubias, A., Trachsel, S., Winkler, R. & Tiessen, A. Metabolic profiling of plant extracts using direct-injection electrospray ionization mass spectrometry allows for high-throughput phenotypic characterization according to genetic and environmental effects. Journal of Agricultural and Food Chemistry 63, 1042–1052 (2015).

23. Treviño, V., Yañez‐Garza, I., Rodriguez‐López, C. E., Urrea‐López, R., Garza‐Rodriguez, M., Barrera‐Saldaña, H., Tamez‐Peña, J. G., Winkler, R. & Díaz de‐la‐Garza, R. GridMass: a fast two‐dimensional feature detection method for LC/MS. Journal of Mass Spectrometry 50, 165–174 (2015).

24. Moreno-Pedraza, A., Valdés-Santiago, L., Hernández-Valadez, L. J., Rodríguez-Sixtos Higuera, A., Winkler, R. & Guzmán-de Peña, D. L. Reduction of aflatoxin B1 during tortilla production and identification of degradation by-products by direct-injection electrospray mass spectrometry (DIESI-MS). Salud Pública de México 57, 50–57 (2015).

25. Winkler, R. SpiderMass: semantic database creation and tripartite metabolite identification strategy. Journal of Mass Spectrometry 50, 538–541 (2015).

26. Sotelo-Silveira, M., Chauvin, A.-L., Marsch-Martínez, N., Winkler, R. & De Folter, S. Metabolic fingerprinting of Arabidopsis thaliana accessions. Frontiers in Plant Science 6, 1–13 (2015).

27. Barraza, A., Cabrera-Ponce, J. L., Gamboa-Becerra, R., Luna-Martínez, F., Winkler, R. & Álvarez-Venegas, R. The Phaseolus vulgaris PvTRX1h gene regulates plant hormone biosynthesis in embryogenic callus from common bean. Frontiers in Plant Science 6, 1–16 (2015).

28. Gamboa-Becerra, R., Ramírez-Chávez, E., Molina-Torres, J. & Winkler, R. MSI. R scripts reveal volatile and semi-volatile features in low-temperature plasma mass spectrometry imaging (LTP-MSI) of chilli (Capsicum annuum). Analytical and Bioanalytical Chemistry 407, 5673–5684 (2015).

29. López-Castillo, L. M., López-Arciniega, J. A., Guerrero-Rangel, A., Valdés-Rodríguez, S., Brieba, L. G., García-Lara, S. & Winkler, R. Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification. Frontiers in Plant Science 6, 1–13 (2015).

30. Winkler, R. An evolving computational platform for biological mass spectrometry: workflows, statistics and data mining with MASSyPup64. PeerJ 3, 1–34 (2015).

31. Winkler, R. MASSyPup—an ‘Out of the Box’solution for the analysis of mass spectrometry data. Journal of Mass Spectrometry 49, 37–42 (2014).

32. Maldonado-Torres, M., López-Hernández, J. F., Jiménez-Sandoval, P. & Winkler, R. ‘Plug and Play’assembly of a low-temperature plasma ionization mass spectrometry imaging (LTP-MSI) system. Journal of Proteomics 102, 60–65 (2014).

33. Ortiz-Martinez, M., Winkler, R. & García-Lara, S. Preventive and therapeutic potential of peptides from cereals against cancer. Journal of Proteomics 111, 165–183 (2014).

34. Valdés, J., Nozaki, T., Sato, E., Chiba, Y., Nakada-Tsukui, K., Villegas-Sepúlveda, N., Winkler, R., Azuara-Liceaga, E., Mendoza-Figueroa, M. S. & Watanabe, N. Proteomic analysis of Entamoeba histolytica in vivo assembled pre-mRNA splicing complexes. Journal of Proteomics 111, 30–45 (2014).

35. Yasui, M. T., Mata‐Gómez, M. A. & Winkler, R. Lysine‐directed staining of proteins for MS‐based analyses. Electrophoresis 34, 401–404 (2013).

36. Montero-Vargas, J. M., González-González, L. H., Gálvez-Ponce, E., Ramírez-Chávez, E., Molina-Torres, J., Chagolla, A., Montagnon, C. & Winkler, R. Metabolic phenotyping for the classification of coffee trees and the exploration of selection markers. Molecular BioSystems 9, 693–699 (2013).

37. Martínez‐Jarquín, S. & Winkler, R. Design of a low‐temperature plasma (LTP) probe with adjustable output temperature and variable beam diameter for the direct detection of organic molecules. Rapid Communications in Mass Spectrometry 27, 629–634 (2013).

38. Bretschneider, T., Heim, J. B., Heine, D., Winkler, R., Busch, B., Kusebauch, B., Stehle, T., Zocher, G. & Hertweck, C. Vinylogous chain branching catalysed by a dedicated polyketide synthase module. Nature 502, 124–128 (2013).

39. Mata-Gómez, M. A., Yasui, M. T., Guerrero-Rangel, A., Valdés-Rodríguez, S. & Winkler, R. Accelerated identification of proteins by mass spectrometry by employing covalent pre-gel staining with Uniblue A. PloS One 7, e31438 (2012).

40. Valdes-Santiago, L., Cervantes-Chavez, J. A., Winkler, R., León-Ramírez, C. G. & Ruiz-Herrera, J. Phenotypic comparison of samdc and spe mutants reveals complex relationships of polyamine metabolism in Ustilago maydis. Microbiology 158, 674–684 (2012).

41. García-Flores, M., Juárez-Colunga, S., Montero-Vargas, J. M., López-Arciniega, J. A. I., Chagolla, A., Tiessen, A. & Winkler, R. Evaluating the physiological state of maize (Zea mays L.) plants by direct-injection electrospray mass spectrometry (DIESI-MS). Molecular BioSystems 8, 1658–1660 (2012).

42. Wartenberg, D., Vödisch, M., Kniemeyer, O., Albrecht-Eckardt, D., Scherlach, K., Winkler, R., Weide, M. & Brakhage, A. A. Proteome analysis of the farnesol-induced stress response in Aspergillus nidulans - The role of a putative dehydrin. Journal of Proteomics 75, 4038–4049 (2012).

43. Tefsen, B., Lagendijk, E. L., Park, J., Akeroyd, M., Schachtschabel, D., Winkler, R., Die, I. van & Ram, A. F. Fungal α-arabinofuranosidases of glycosyl hydrolase families 51 and 54 show a dual arabinofuranosyl-and galactofuranosyl-hydrolyzing activity. Biologial Chemistry 393(8), 767–775 (2012).

44. Garcıa-Flores, M., Juarez-Colunga, S., Montero-Vargas, J. M., Lopez-Arciniega, J. A. I., Chagolla, A., Tiessena, A. & Winkler, R. Evaluating the physiological state of maize (Zea mays L.) plants by direct-injection electrospray mass spectrometry (DIESI-MS) w. Molecular BioSystems 8, 1658–1660 (2012).

45. Burmester, A., Shelest, E., Glöckner, G., Heddergott, C., Schindler, S., Staib, P., Heidel, A., Felder, M., Petzold, A., Szafranski, K., Feuermann, M., Pedruzzi, I., Priebe, S., Groth, M., Winkler, R., Li, W., Kniemeyer, O., Schroeckh, V., Hertweck, C., Hube, B., White, T. C., Platzer, M., Guthke, R., Heitman, J., Wöstemeyer, J., Zipfel, P. F., Monod, M. & Brakhage, A. A. Comparative and functional genomics provide insights into the pathogenicity of dermatophytic fungi. Genome Biology 12, R7 (2011).

46. Vödisch, M., Scherlach, K., Winkler, R., Hertweck, C., Braun, H.-P., Roth, M., Haas, H., Werner, E. R., Brakhage, A. A. & Kniemeyer, O. Analysis of the Aspergillus fumigatus proteome reveals metabolic changes and the activation of the pseurotin A biosynthesis gene cluster in response to hypoxia. Journal of Proteome Research 10, 2508–2524 (2011).

47. González-González, M., Mayolo-Deloisa, K., Rito-Palomares, M. & Winkler, R. Colorimetric protein quantification in aqueous two-phase systems. Process Biochemistry 46, 413–417 (2011).

48. Rawat, R., Sandoval, F. J., Wei, Z., Winkler, R. & Roje, S. An FMN hydrolase of the haloacid dehalogenase superfamily is active in plant chloroplasts. Journal of Biological Chemistry 286, 42091–42098 (2011).

49. Fries, A., Bretschneider, T., Winkler, R. & Hertweck, C. A Ribonucleotide Reductase‐Like Electron Transfer System in the Nitroaryl‐Forming N‐Oxygenase AurF. ChemBioChem 12, 1832–1835 (2011).

50. Kiehntopf, M., Schmerler, D., Brunkhorst, F. M., Winkler, R., Ludewig, K., Osterloh, D., Bloos, F., Reinhart, K. & Deufel, T. Mass Spectometry-Based Protein Patterns in the Diagnosis of Sepsis/Systemic Inflammatory Response Syndrome. Shock 36, 560–569 (2011).

51. Winkler, R. ESIprot: a universal tool for charge state determination and molecular weight calculation of proteins from electrospray ionization mass spectrometry data. Rapid Communications in Mass Spectrometry 24, 285–294 (2010).

52. García-Lara, S. & Winkler, R. Activity-directed identification of maize kernel peroxidases associated with postharvest insect resistance. Molecular BioSystems 6, 1810–1812 (2010).

53. Fries, A., Winkler, R. & Hertweck, C. Structural and biochemical basis for the firm chemo-and regioselectivity of the nitro-forming N-oxygenase AurF. Chemical Communications 46, 7760–7762 (2010).

54. Vödisch, M., Albrecht, D., Leßing, F., Schmidt, A. D., Winkler, R., Guthke, R., Brakhage, A. A. & Kniemeyer, O. Two‐dimensional proteome reference maps for the human pathogenic filamentous fungus Aspergillus fumigatus. Proteomics 9, 1407–1415 (2009).

55. Meiller, T. F., Hube, B., Schild, L., Shirtliff, M. E., Scheper, M. A., Winkler, R., Ton, A. & Jabra-Rizk, M. A. A novel immune evasion strategy of Candida albicans: proteolytic cleavage of a salivary antimicrobial peptide. PloS One 4, 1–9 (2009).

56. Schmaler-Ripcke, J., Sugareva, V., Gebhardt, P., Winkler, R., Kniemeyer, O., Heinekamp, T. & Brakhage, A. A. Production of pyomelanin, a second type of melanin, via the tyrosine degradation pathway in Aspergillus fumigatus. Applied and Environmental Microbiology 75, 493–503 (2009).

57. Melle, C., Ernst, G., Winkler, R., Schimmel, B., Klussmann, J. P., Wittekindt, C., Guntinas‐Lichius, O. & Eggeling, F. von. Proteomic analysis of human papillomavirus‐related oral squamous cell carcinoma: Identification of thioredoxin and epidermal‐fatty acid binding protein as upregulated protein markers in microdissected tumor tissue. Proteomics 9, 2193–2201 (2009).

58. Lackner, G., Möbius, N., Scherlach, K., Partida-Martinez, L. P., Winkler, R., Schmitt, I. & Hertweck, C. Global distribution and evolution of a toxinogenic Burkholderia-Rhizopus symbiosis. Applied and Environmental Microbiolog 75, 2982–2986 (2009).

59. Schmitt, I., Partida-Martinez, L. P., Winkler, R., Voigt, K., Einax, E., Dölz, F., Telle, S., Wöstemeyer, J. & Hertweck, C. Evolution of host resistance in a toxin-producing bacterial–fungal alliance. The ISME Journal 2, 632–641 (2008).

60. Winkler, R. Biosynthese von Nitroverbindungen. Biospektrum 14, 434 (2008).

61. Winkler, R. & Hertweck, C. Biosynthesis of nitro compounds. ChemBioChem 8, 973–977 (2007).

62. Winkler, R., Zocher, G., Richter, I., Friedrich, T., Schulz, G. E. & Hertweck, C. A Binuclear Manganese Cluster That Catalyzes Radical‐Mediated N‐Oxygenation. Angewandte Chemie International Edition 46, 8605–8608 (2007).

63. Zocher, G., Winkler, R., Hertweck, C. & Schulz, G. E. Structure and Action of the N-oxygenase AurF from Streptomyces thioluteus. Journal of Molecular Biology 373, 65–74 (2007).

64. Teichmann, K., Winkler, R., Hampel, K., Trümpler, A., Böhmer, F. D. & Imhof, D. Monitoring phosphatase reactions of multiple phosphorylated substrates by reversed-phase HPLC. Journal of Chromatography B 853, 204–213 (2007).

65. Winkler, R., Richter, M. E., Knüpfer, U., Merten, D. & Hertweck, C. Regio‐and Chemoselective Enzymatic N‐Oxygenation In Vivo, In Vitro, and in Flow. Angewandte Chemie International Edition 45, 8016–8018 (2006).

66. Winkler, R. & Hertweck, C. Sequential enzymatic oxidation of aminoarenes to nitroarenes via hydroxylamines. Angewandte Chemie International Edition 44, 4083–4087 (2005).

67. Reiber, K., Reeves, E. P., Neville, C. M., Winkler, R., Gebhardt, P., Kavanagh, K. & Doyle, S. The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron. FEMS Microbiology Letters 248, 83–91 (2005).